BD 253020 containing: one 4.5 mL bottle Extraction Reagent A , one 7 mL bottle Wash Reagent 1 , one 3.5 mL bottle Detector Reagent 2 , one 13.5 mL bottle Wash Reagent 3 , one 7 mL bottle Substrate A, Reagent 4 , one 7 mL bottle Substrate B, Reagent 5, one